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2014 Month : October Volume : 3 Issue : 55 Page : 12602-12611

A COMPARATIVE STUDY ON DIFFERENT PHENOTYPIC METHODS FOR DETECTION OF METALLO BETA LACTAMASE PRODUCING BACTERIA IN A TERTIARY HOSPITAL OF EASTERN INDIA

Anirban Bhaduri1, Rajdeep Saha2, Papiya Biswas3, Suranjana Mitra4, Sharmistha Datta (Basu)5, Paramita Das6, Sougata Ghosh7, Manas Sarkar8

CORRESPONDING AUTHOR:
Dr. Anirban Bhaduri,
22/1 RAK Road,
Dum Dum,
Kolkata-55.
Email: anir8365@gmail.com

ABSTRACT: BACKGROUND: Metallo beta lactamase (MBL) producing bacteria are popularly known as superbugs due to their increased development of drug resistance to carbapenems. MBL producing bacteria mainly cause healthcare associated infections (HAI) and are the causes of increased mortality among patients infected with MBL producers. The aim of this study was to compare the different phenotypic methods available for detecting MBL producing bacteria. MATERIALS AND METHODS: 500 samples from different clinical specimens were processed and samples from which gram negative bacilli was isolated were screened for imipenem resistance by Kirby –bauer disc diffusion methods and further tested for MBL detection by Imipenem+EDTA combined disk diffusion method, Imipenem+EDTA double disk synergy test and MBL E-test. RESULTS AND ANALYSIS: Out of the 125 strains of GNB, 32 strains were found to be Imipenem resistant by Kirby Bauer disk diffusion, 18 (56.25%) were Pseudomonas aeruginosa, 7 (21.87%) were K.pneumoniae, 4 (12.5%) were Acinetobacter spp, 2 (6.25%) were Citrobacter and 1 (3.12%) was E.coli. When these 32 isolates were tested for MBL production by IMP+EDTA Combined disk diffusion test, 29 (90.62%) of the isolates were found to be MBL producers, by Imipenem+EDTA double disk synergy test, 26(81.25%) of the isolates showed MBL production and by MBL E-test, 30 (93.75%) of the isolates were MBL producers.

CONCLUSION: From our study it appears that metallo- β- lactamase production in gram negative bacilli has become a significant problem in health care associated infection control in this part of India and is producing many carbapenemase resistant strains in India.

KEYWORDS: Metallo beta lactamase, Health care associated infections, E-test.

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